Development of Chinese Near-isogenic Line of Rice and Their Differentiating Ability of Pathogenic Races of Blast Fungus

The rice( Oryza L. ) variety, Lijiangxintuanheigu(LTH), is a universally susceptible variety. A set of near-isogenic lines (NILs) of rice which has single resistance gene for each line was developed by use of LTH as recurrent parent and Kusabue,Tsuyuake, K1, PiNo. 4, K60 and BL1, which are from Kiyosawa‘s differential varieties(KDV), as donor parents. Differentiating ability of these NILs to Philippine isolates was compared with that of their donor parents, KDV and IRRI‘ s NILs. The results of comparative studies were as follows: (1) Differentiating ability of Chinese NILs to Philippine isolates was much stronger than that of their donor parents and that of KDV. (2) Chinese NILs had similar ability to differentiate races to IRRI‘s NILs or higher differentiating ability than them. But IRRI‘s NILs almost had no differentiating ability to isolates from japonica rice-growing regions. They can not be used to differentiate races of isolates from japonica ricegrowing regions. Whereas, Chinese NILs can be used to differentiate races of isolates from both japonica ricegrowing regions and indica rice-growing regions. It was concluded that the set of Chinese NILs can be used as international differentials.     

陆地棉核雄性不育材料在山东不同地区的育性转变及应用研究

对在山东的济南、平度、莱西、烟台种植的几种陆地棉（Ｇｏｓｓｙｐｉｕｍ　ｈｉｒｓｕｔｕｍＬ．）核雄性不育材料的育性表现进行了研究,结果表明：洞Ａ不育系的衍生材料ＭＢ、ＭＡ在不同气候条件下有育性的变化。在济南高度不育,可作为不育系。在其他几个地区表现不同程度的可育。其中ＭＢ在平度有相当好的育性,能正常结铃。而双隐性核不育材料５９Ａ、６２Ａ未能观察到育性变化。本文就核不育材料在棉花杂交制种中的应用前景及需深入研究的问题作了探讨。     

Inhibitory effects of LDL-ACM complex on subcutaneous implanted tumors in nude mice()

AIM: In order to evaluate the applicable value of LDL as a targeted vehicle for chemotherapeutic agents, we investigated and compared the inhibitory effects of LDL-ACM complex and free ACM on nude mice's subcutaneous implanted tumors derived from gastric cancer cell lines, SGC-7901 and NKM-45. METHODS: LDL-ACM complex was prepared and the tumor model of nude mice was established by subcutaneous implantation of SGC-7901 and NKM-45. Then, the groups of nude mice developed subcutaneous implanted tumors were received either LDL-ACM complex or free ACM. Subsequently, the tumor size, weight and leukemia cell counts were measured and the rates of tumor-inhibition and the survival were compared among the groups. RESULTS: The inhibitory effects of LDL-ACM complex on the tumors, especially on SGC-7901 implanted tumors were much more obvious than that of free ACM. It was also indicated that the action of LDL-ACM complex was mediated by LDL receptor. CONCLUSION: These results showed that LDL-ACM complex had significant inhibitory effects on the implanted tumors and the effect might be mediated by LDL receptor.     

大白菜细胞核雄性不育基因向自交系97A407的转育

根据复等位基因遗传假说 ,利用大白菜核不育系 9810 9和已知基因型的甲型两用系可育株 99135 2作为不育源 ,以大白菜自交系 97A40 7为目标亲本 ,经过 5个世代的转育 ,获得了具有 97A40 7遗传基因型的新甲型两用系、临时保持系和雄性不育系。     

人血小板生成素基因的克隆及CHO稳定细胞系的建立

 以人肝cDNA为模板克隆了人血小板生成素（Human Thrombopoietin,hTPO）基因,利用基因重组技术构建了带有新霉素抗性基因（neo）筛选标记的pcDNA3.1(+)-hTPO真核表达载体,将重组质粒瞬时转染293T细胞,用鼠抗人TPO 单抗Western blot 检测 TPO蛋白的瞬时表达;再将重组质粒转染中国仓鼠卵巢细胞(Chinese Hamster Ovary,CHO),应用400 μg/mL的G418筛选克隆,经PCR及Western blot验证,获得了3株hTPO蛋白表达水平不同的CHO细胞系,为获得大量蛋白并进行活性功能试验及临床应用奠定基础。     

高温不育小麦育性转换特性研究

为研究高温雄性不育小麦周13S -1的育性转换特性,2004～2005年在广东湛江进行了分期播种试验.结果表明,周13S -1育性变化趋势为"部分不育-高度不育-部分不育",不育的时间长,不育期败育程度高.温度是引起周13S -1雄性育性发生转换的主要原因,日长对其雄性育性没有显著影响.抽穗前26～18 d平均日均温与自交结实率的相关达极显著水平.当抽穗前25～21 d平均日均温在20.32℃以上时周13S -1表现完全不育;低于19℃表现部分不育,能够自交结实,且育性随着温度的降低而升高.说明周13S -1的育性临界温度在20℃左右.在湛江10月15日至11月15日播种表现高度不育,自交结实率在1.5%以下,可以用于生产杂交种;10月15日以前和11月15日以后播种均表现部分不育,可以自交繁殖.育性转换对温度的敏感时期为抽穗前26～18 d,此时生育时期为倒二叶露尖前后,穗分化时期为药隔期.相关分析表明花粉育性和自交结实率无显著相关.     

优良糯玉米自交系lz-8的选育与应用

lz-8-5-2-2是以贵州省一糯质农家种为材料,经连续多代自交分离选育而成,具有优质、高配合力等特点。以其为亲本育成了优良糯玉米新品种柳糯582、柳糯8号,并通过了品种审定,值得推广种植。     

低硝酸盐含量萝卜的筛选

对萝卜4个雄性不育系杂交组合和10个商品种的F1代及F2代进行硝酸盐含量测定分析。结果表明,萝卜雄性不育系杂交组合F1代与其母本雄性不育系的硝酸盐含量范围相近,说明母本雄性不育系对F1代的硝酸盐含量有决定作用。10个商品种F1代的硝酸盐含量均较高,且品种间差异显著;其F2代产生剧烈分离,变异系数为11．46％-44．23％,品系内硝酸盐含量的变化范围也随之扩大,出现了F1代品种内没有的低硝酸盐含量和高硝酸盐含量单株。显示出利用F1代自交分离筛选低硝酸盐含量萝卜株系的可行性。     

印楝种籽初制工艺技术装备的研制

本文对印楝种籽的加工机理进行了研究,提出了满足生产需要的工艺路线,研制出了差速螺旋齿双级对滚破壳设备、吸风分离设备,获得了较好的加工效果。     

优质杂交晚籼稻新组合乐优97的选育及栽培制种技术

乐优97是一种典型的晚籼型三系杂交水稻良种,于2022年通过中国国家农作物品种审定委员会审定,系由合肥丰乐种业股份有限公司所属的水稻研究院通过自己创制的三系不育系409A与恢复系R977 杂交选育而成的高产、优质、抗倒杂交晚籼型新型组合。文中详细尽介绍了乐优97的选育步骤,重点介绍了乐优97的特征特性,并归纳出了乐优97优质高产栽培技术及其制种技术关键要点,希望为该产品的以后大规模推广种植打下基础。